多变鱼腥藻半稳定状态连续培养的若干特征

SOME CHARACTERISTICS OF THE CONTINUOUS CULTURE OF AN ABAEN A V ARIABILIS (BLUE-GREEN ALGA, CYANOBACTERIUM) IN SEMI-STEADY STATE

分别使用恒化装置和全玻璃发酵罐连续培养多变鱼腥藻。在稳定条件下加以周期性的光照(L∶D=12∶12),经5—6d得到半稳定状态连续培养物。在光照阶段,培养物的生物量增长速度约等于稀释速度D的两倍,叶绿素的增长速度略小于D的两倍,糖原的累积速度几乎高出D^5的一个数量级。细胞内糖原和蛋白质含量变化的时间过程反映出培养物在光-暗条件下的碳氮物质合成与消耗相互联系的代谢特点。半稳定状态连续培养受培养物内在的生理活性支配,生长参数的变化符合方程X=X_(oe)^((μ-D)~t),但不同于恒浊培养和恒化培养。外界因素的强弱变化只改变生长参量变化的强度。本法适用于研究微藻的生态生理。

A chemostat apparatus and a whole glass fermentor were used in the continuous culture of Anabaena vasriabilis. When diurnal light-dark cycles (12:12 h) were incorporated in the stable conditions of the cultivation, a semi-steady state of the culture was achieved in 5—6 days. The periodic change rates of biomass were different from those of other growth parameters. The metabolic features of the culture under the L:D cycles could be i licated by the comparative data on the synthesis and accumulation of cell components (e.g., glycogen and protein) over the time course. The growth of the culture showed an innate physiological regularity. Changes of the growth parameters can be described by the equation X=X_(oe)^(μ-D)t. This semi-steady state of continuous culture was different from chemostatic and turbidostatic cultures. The technique is suitable for the research of algal ecophysiology and algal biotechnology.