摘要
AIM: To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver.METHODS: Peripheral blood mononuclear cells were collected in 56 patients with fatty liver, 26 patients without fatty liver and 20 normal controls. Then PCR-RFLP was used to analyze genetic polymorphism of CYPIIE1 in monocytes on the region of Pst I and Rsa I.RESULTS: The frequency of homozygotic C1 gene in patients with alcoholic fatty liver (28.6 %), obese fatty liver (38.5 %), or diabetic fatty liver (33.3 %) was significantly lower than that of the corresponding patients without fatty liver (100 %, 100 % and 80 % respectively), while the frequency of C2 genes, including C1/C2 and C2/C2, was significantly higher (71.4 %/0 %, 61.5 %/0 %, and 66.7 %/20 %) (P<0.01). The frequency distribution of the above genes of non-fatty liver patients (100 %/0, 100 %/0, and 80 %/20 %) was not significantly different from that of the normal controls (85 %/15 %) (P>0.05).CONCLUSION: The genetic polymorphism of CYPIIE1 on the position of Pst I and Rsa I is related to the susceptibility of fatty liver. Besides, C2 gene may play a key role in the pathogenesis of fatty liver.
AIM:To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver. METHODS:Peripheral blood mononuclear cells were collected in 56 patients with fatty liver,26 patients without fatty liver and 20 normal controls.Then PCR-RFLP was used to analyze genetic polymorphism of CYPIIE1 in monocytes on the region of Pst Ⅰ and Rsa Ⅰ. RESULTS:The frequency of homozygotic C1 gene in patients with alcoholic fatty liver (28.6 %),obese fatty liver (38.5 %),or diabetic fatty liver (33.3 %) was significantly lower than that of the corresponding patients without fatty liver (100 %,100 % and 80 % respectively),while the frequency of C2 genes,including C1/C2 and C2/C2,was significantly higher (71.4 %/0 %,61.5 %/0 %,and 66.7 %/ 20 %) (P<0.01).The frequency distribution of the above genes of non-fatty liver patients (100 %/0,100 %/0,and 80 %/20 %) was not significantly different from that of the normal controls (85 %/15 %) (P>0.05). CONCLUSION:The genetic polymorphism of CYPIIE1 on the position of Pst Ⅰ and Rsa Ⅰ is related to the susceptibility of fatty liver.Besides,C2 gene may play a key role in the pathogenesis of fatty liver.
