减蛋综合征病毒PVⅢ蛋白基因的扩增克隆与鉴定

Amplified cloning and identification of PVⅢ gene of egg drop syndrome virus

应用降落PCR法对减蛋综合征病毒PVⅢ蛋白基因进行了扩增 ,结果得到 1.1kb的扩增产物。将该扩增产物克隆至 pMD18-T载体中 ,经酶切和PCR鉴定后 ,证明了目的片段的正确性 ,从而实现了EDSV -gDNAPVⅢ基因的克隆 ,这不仅为进一步阐明EDSVPVⅢ结构与功能的关系打下了基础 ,而且为PVⅢ基因的表达及EDS基因工程苗的研究也奠定了良好的基础。

PVⅢ protein gene of egg drop syndrome virus (EDSV) was amplified by touchdown PCR(TD-PCR) and was cloned into pMD18-T vector. The fragment was 1.17kb . The recombinant plasmids were identified by digested with restriction endonuclease and PCR. The results will lay a sound foundation for further study on the relationship between structure and function of PVⅢ gene of EDSV-gDNA .