5个美国扁桃品种的微繁殖

Micropropagation of 5 Almond Cultivars Introduced from U.S.A

对5个引进的美国扁桃品种Bulgaria1、Nonpareil、Fritz、Prize和Neplus的微繁殖进行了研究。以单芽茎段作为外植体,所用启动培养基为MS+0.5～1.0mg/LBA+0～0.1mg/LIAA,增殖培养基为MS+0.5～2.0mg/LBA+0.1mg/LIAA+0.5mg/LGA3,壮苗培养基为MS+0.1mg/LBA+162.14mg/LPG。生根培养方法为100mg/LIBA溶液浸渍60min后接入无任何生长激素的1/2MS上暗培养2周,或在1/2MS+100mg/LIBA+200mg/L柠檬酸上培养12h转入无任何生长激素的1/2MS上暗培养8d。采用试管苗基部遮光可代替暗培养,但生根率略低而地上部分生长更健壮。

Studies on the micropropagation of5cultivars of almond,Bulgaria1,Nonpareil,Fritz,Prize and Neplus,was conducted with node explants.Initiation medium(MS+0.5～1.0mg /L BA+0～0.1mg /L IAA),multiplication medium(MS+0.5～2.0mg /L BA+0.1mg /L IAA+0.5mg /L GA 3 ),and elongation medium(MS+0.1mg /L BA+162.14mg /L PG)were used.Two in vitro rooting methods,dip in100mg /L IBA for60min followed by dark culture on1/2MS without any growth regulator for first two weeks and culture on1/2MS+100mg /L IBA+200mg /L citric acid for12hr then transferred onto1/2MS without any growth regulator in darkness for first8days,were most effective.Dark culture can be replaced by in vitro basal darkening method but the rooting percentage was lower and shoots were healthier and stronger.