鸡胰岛素样生长因子-1基因的遗传多态性与其体重的关系

Relationship between the Polymorphisms of IGF-Ⅰ Gene and Body Weight in Chicken Breeds

对宁都三黄鸡、万载康乐黄鸡两个鸡种共295个样本,采用PCR RFLP技术对胰岛素样生长因子—1(cIGF 1)基因的5'端调控区进行遗传变异研究,发现该调控区的扩增产物经PstⅠ酶切后出现3种基因型(AA、AB、BB)。结果表明:性别对宁都三黄鸡这3种基因型的分布呈现显著差异(P<0 05)。分析这3种基因型与其体重的关系发现,这3种基因型对初生重、30日龄体重、60日龄体重、90日龄体重及120日龄体重影响差异均不显著(P>0 05),但体现出AA型有较高体重的趋势,公母表现出一致的规律。在万载康乐黄鸡中,均呈现出杂合优势,在8周龄及18周龄时差异不显著(P>0 05),而在12周龄表现为差异显著(P<0 05)。宁都三黄鸡公母基因型分布均符合哈代 温格伯定律,而万载康乐黄鸡基因型分布不符合哈代 温格伯定律。

Genetic variation of the 5' region of the IGFⅠ gene in Ningdu and Wanzai kangle yellow chicken breeds were detected by means of PCRRFLP.There were three genotypes(AA,AB,BB) after the PCR products were cut by Pst Ⅰ.The results showed that sex significantly affect the distribution of the genotypes(P<005).It was not significant between genotypes and body weight at 1?30?60?90?120 days of ages in Ningdu chickens (P>005),but body weight of AA was slightly more than that of AB and BB,As the same time,there is the same results between female and male.In Wanzai kangle chicken,It was not significant between genotypes and body weight at 8 and 18 week of age,but body weight of AB was slightly more than that of AA and BB (P>005),body weight of AB at 12 weeks was significantly higher than that of AA and BB (P<005).The locus of IGF1 gene was at the HardyWeinberg equilibrium (P>005) in Ningdu male and female chickens,but not in Wanzai Kangle chickens (P<005).