急性感染肝片吸虫水牛血中几种花生四烯酸代谢物及激素动态变化

Changes of Some Blood Arachidonic Acid Metabolites and Hormones in Water Buffaloes Infected Acutely with F.Hepatica

选择12头健康、2～3岁、体重300～400kg雄性去势水牛,粪便检查和Dot—ELISA检测确认无肝片吸虫感染,随机分成感染组(n=9)和对照组(n=3)。感染组每头经口一次性感染1600个肝片吸虫囊蚴。研究了水牛急性感染肝片吸虫后血中几种花生四烯酸代谢物及激素动态变化。结果表明:水牛在急性感染肝片吸虫后整个实验期里没有明显的临床症状,具有较大的耐受力和抵抗力;水牛感染后,PGE2在16周前高于或明显高于对照组,以后两组相近;6 keto PGF1α在整个实验期感染组与对照组无明显差异;TXB2在第1-10周低于或明显低于对照组。血浆Cort在第4-10周高于或明显高于对照组;T4在第4-22周总体水平高于或显著高于对照组;Ins和T3在整个实验期两组比较未见明显差异性变化。结果提示:水牛在急性感染肝片吸虫后,血中一些花生四烯酸代谢物变化明显,参与病理生理发生发展过程。

Twelve healthy male testictomy water buffaloes of 2～3 years old with 300～400kg body weight as well as no fasciolosis confirmed by fecal examination and DotELISA were chosen and randomly divided into infection group (n=9) and control group (n=3).Each buffalo of infection group was orally dosed with 1600 F.heptica metacercariae once.Changes of some blood arachidonic acid metabolites and hormones in water buffaloes infected acutely with F.Hepatica was studied.The results showed that water buffaloes had no observable clinical symptoms during whole experiment period after infecting acutely massive F.hepaticaw with powerful tolerance and defence of themselves.Plasma PGE2 in infection animals were superior or significantly superior to control group during 16 weeks,and then approached the same between both groups;No significant differences of 6ketoPGF1α were investigated as compared with control group during whole experiment;TXB2 levels were inferior or obviously less to control group during 1-10 weeks of postinfection.Plasma cortisol surpassed or did significantly control group during 4-10 weeks;The whole levels of T4 was high or markedly high to control group;there were no obvious changes in insulin and T3 of infection animals compared with control group.It was suggested that markedly changes of some arachidonic acid metabolites participate in pathophysiological development process in blood of water buffaloes infected acutely with F.hepatica.