肺气肿肺气虚证大鼠血气分析、血中细胞因子及肺组织病理学的改变

Changes of Blood-Gas Analysis ,Cytokines and Pulmonary Tissue Pathology in Emphysema Rats with Lung Qi Deficiency Syndrome

目的 :探索肺气虚证的本质。方法 :将 6 0只大鼠随机分为模型组与对照组 ,复制肺气肿肺气虚证模型 ,分别检测 pH、氧分压 (pO2 )、氧饱和度 (SaO2 % )、二氧化碳分压 (pCO2 )、白细胞介素 6 (IL 6 )、白细胞介素 8(IL 8)、肿瘤坏死因子 α(TNF α)及肺组织病理学的改变。结果 :模型组pH、pO2 、SaO2 %下降 ,pCO2 升高 ,与对照组比较 ,差异有显著性 (P <0 .0 1 ) ;模型组IL 6、IL 8、TNF α升高 ,与对照组比较 ,差异有显著性 (P <0 .0 1 ) ;病理组织学及超微结构检查显示肺组织有慢性支气管炎及肺气肿病变。结论 :肺气肿肺气虚证大鼠存在低氧血症和高碳酸血症 ,血中细胞因子IL 6、IL 8、TNF α升高 ,可能是肺气虚证从轻度向中、重度发展的重要原因之一。

Objective:To probe into the essence of lung Qi deficiency syndrome.Method:Sixty rats were randomly divided into control group and model group.The pH,partial pressure of oxygen(p O 2 ),arterial oxygen saturation(SaO 2%),partial pressure of carbon dioxide(p CO 2 ),interleukin 6(IL 6),interleukin 8(IL 8) and tumor necrosis factor α(TNF α) in artery blood and the pulmonary tissue pathology were determined. Result: In model group, the pH,p O 2 and SaO 2% in artery blood were significantly lower than those in control group (P<0.01),while the p CO 2 ,IL 8 and TNF α were significantly higher than those in control group(P<0.01). The pathology examination of pulmonary tissue showed pulmonary emphysema and chronic bronchitis. Conclusion:Rats with Lung Qi deficiency syndrome have lower oxygen ,lower hypercapnia, higher IL 6, higher IL 8 and higher TNF α,which perhaps is one of the importance factors that lung Qi deficiency syndrome aggravates gradually.