CC16基因G38A突变与汉族哮喘相关性的研究

Study on association between CC16 gene G38A mutation and asthma in the patients of Han population in Chongqing, China

目的 检测中国重庆汉族人群 CC16基因第 1外显子突变在支气管哮喘和正常人群中的频率 ,探讨该基因的多态性和哮喘的相关性。方法 应用聚合酶链反应和限制性片段长度多态性技术对 5 0例支气管哮喘患者 CC16第 1外显子基因频率和等位基因频率进行检测并与 5 0例正常人进行对照。结果CC16基因第 1外显子 G38A的基因型和等位基因型频率在哮喘组和对照组差异无显著性 ,并且该基因的基因型频率和等位基因型频率与哮喘病情的严重程度也无相关性。 结论 中国重庆汉族人群中 CC16第 1外显子 G38A的突变与哮喘无相关性。

Objective To investigate the possible association between polymorphism of CC16 gene exon 1 and asthma, the genotype and allele frequencies of CC16 gene exon 1 in the asthmatic patients of Han population in southwest China were analyzed. Methods The authors determined the genotypes of CC16 gene exon 1 with polymerase chain reaction technique and restricted enzyme analysis, and then compared the genotype and allele frequencies of the gene of the asthmatic group with those of the healthy control group. Results There was no significant difference in genotype and allele frequencies of CC16 gene between the asthmatic group and control group.There was no association between the genotype and allele frequencies of gene and the severity of asthma. Conclusion CC16 gene may be not a susceptibility gene of asthmatic patients of Han population in southwest China.