丹皮酚提取、磺化及对植物病原菌的抑菌试验

Experiment on Extraction,Sulfonate of Paeonol and Its Antibiotic Effect on Plant Pathogen

测定安徽南陵地区不同生长年限的牡丹根皮、木心及茎、叶的丹皮酚含量 ,并比较不同提取方法的效果 ;丹皮酚用浓硫酸和发烟硫酸磺化 ,得产物丹皮酚磺酸钠 ,并通过HPLC、TLC、FTIR、UV检测鉴定其理化性质 ;用丹皮酚、丹皮酚磺酸钠进行了初步的抑制植物病原菌试验 ,发现对RS10 5、青枯病菌、苹果斑枯病菌、玉米纹枯病菌有较强的抑制作用 ,并对玉米苗期纹枯病显示出较好的防治效果。

Paeonol contents in root barks, cores,stems and leaves of Paeonia suffruticosa Andr. from Nanling county,Anhui province were determined and paeonol was extracted with alcohol,acid alcohol and alkali alcohol. Sodium paeonol sulfonate was synthesized by treating paeonol with concentrated sulfuric acid and oleums,and its physicochemical properites were surveyed by HPLC, TLC, FTIR and UV. Antibiotic experiment on plant pathogenic fungi and bacteria showed that paeonol and sodium paeonol sulfonate had remarkably inhibitive effects on Xanthmonas oryzae pv. oryzicola , Pseudomonas solanacearum , Phyllosticta mali and Rhizoctonia solani . Paeonol and sodium paeonol sulfonate had antibiotic effect on maize sheath blight.