苯甲酸和苯乙酸对松材线虫及细菌活性的影响

Activity Determination of Benzoic Acid and Phenylacetic Acid Against Bursaphelenchus xylophilus and Bacteria

在离体条件下,测定了苯甲酸(BA)和苯乙酸(PA)对马尾松(Pinusmassoniana)水培枝叶、松材线虫(Bursaphelenchusxylophilus)及对分离自松材线虫虫体和健康马尾松的细菌菌株GD1和GD2的作用。结果显示,BA和PA对马尾松离体松叶、松枝均具有毒性,其中BA的毒性较强;一定浓度的BA能增强松材线虫的活力,低浓度BA和较高浓度PA均能促进松材线虫的繁殖,低浓度PA能促进菌株GD1的繁殖;高浓度BA和高浓度PA均能抑制松材线虫的活力,其中BA的抑制活性较强;高浓度BA能抑制松材线虫的繁殖,高浓度BA和较高浓度PA均能抑制菌株GD1的繁殖,而BA和PA对菌株GD2繁殖的抑制活性较对菌株GD1的强。

Pine wood nematode disease is a most devastating disease of pine trees.In order to make clear the pathogenic mechanism of the disease, under in vitro condition,the activity of benzoic acid(BA) and phenylacetic acid(PA) against the aquacultural excised needles and branches of Pinus massoniana(Pm),Bursaphelenchus xylophilus(Bx) and the diseaserelated bacterial strain GD1(isolated from the body of Bx) and bacterial strain GD2(isolated from the healthy Pm),was determined.The results showed that both BA and PA were toxic to the excised needles and branches of Pm,and the toxicity of BA was stronger.BA of certain concentration(100 μg/mL) could strengthen the vitality of Bx.Both BA of low concentration(10 μg/mL) and PA of higher concentration(100,1 000 μg/mL) could stimulate the reproduction of Bx.PA of low concentration(10 μg/mL) could stimulate the multiplication of GD1.Both BA of high concentration(1 000 μg/mL) and PA of high concentration(1 000 μg/mL) could inhibit the vitality of Bx,and BA had a stronger inhibition activity.BA of high concentration(1 000 μg/mL) could inhibit the reproduction of Bx.Both BA of high concentration(1 000 μg/mL) and PA of higher concentration(100,1 000 μg/mL) could inhibit the multiplication of GD1,while the inhibition activity of both BA and PA against the multiplication of GD2 was stronger than against that of GD1.