葛根素口服制剂抗心肌缺血及对心肌细胞的保护作用

Protective effect of Puerarin Oral Formulation on myocardial ischemia in rats and on cardiomyocytes of neonatal rats during hypoxia

目的 研究葛根素口服制剂对在体大鼠心肌和离体新生大鼠心肌细胞的保护作用。方法 实验大鼠随机分为空白对照组、阳性对照组 (造模前尾 iv 0 .0 335 g/ kg葛根素注射液 )和葛根素口服制剂高、低剂量组 ( ig 0 .8,0 .4 g/ kg,连续 6 d) ,以 1U/ kg垂体后叶素 ( Pit)诱导大鼠心肌缺血 ,记录尾 iv Pit前及注射后 5 ,15 ,30 s和 2 ,5 ,10 min大鼠心电图变化 ;显微镜观察心肌细胞形态学变化及测定不同时间点培养液中乳酸脱氢酶 ( L DH)、肌酸激酶 ( CK)和α-羟丁酸脱氢酶 (α- HBDH )的活性。结果 葛根素口服制剂 ig给药对心肌缺血大鼠的心电图 T波、P- R间期、Q- T间期变化有明显的改善 ;能对抗缺氧状态下离体新生大鼠心肌细胞形态学和酶学的异常变化。

Object To study the protective effect of Puerarin Oral Formulation (POF) on myocardial ischemia in rats and on cardiomyocytes of neonatal rats during hypoxia. Methods The experimental rats were randomly divided into the control group, the positive group (iv 0.033 5 g/kg Purerarin Injection before myocardial ischemia) and two POF groups (ig 0.8, 0.4 g/kg for 6 days continualy), myocardial ischemia rats were induced by posterior pituitary Pit injection. The normal ECG and the changed ECG of rats at 5, 15, 30 s, 2, 5, 10 min after giving Pit were recorded; The changes of cardiomyocytes form of neonatal rats in all groups were observed with microscope and the activity of LDH, CK, α HBDH in cell culture medium were assayed. Results Compared with the ECG of rats in control group, T wave, P R and Q T of ECG of rats in the treatment groups had significant difference; The activity of LDH, CK, α HBDH were inhibited by POF. Conclusion Myocardial ischemia caused by Pit in rats could be inhibited by POF. It shows that POF has protective effect on cardiomyocytes of neonatal rats during hypoxia.