RP-HPLC法测定密蒙花中毛蕊花苷的含量

Quantitative determination of verbascoside in Buddleja officinalis by RP-HPLC

目的 测定密蒙花中毛蕊花苷的含量 ,为药材及其制剂的质量控制提供依据。方法 采用 RP- HPL C法测定药材中毛蕊花苷含量。色谱条件 :Inertsil ODS- 3C1 8色谱柱 ( 5μm,2 5 0 m m× 4 .6 m m) ;流动相 :甲醇 -乙腈 - 1%醋酸水溶液 ( 8∶ 16∶ 76 ) ;检测波长 :2 5 4 nm。通过正交实验确定最优提取方案为 :2 0倍量的 70 %乙醇回流提取 1.0h。结果 该分析方法可以使样品达到基线分离 ,毛蕊花苷的保留时间约 14 min。该方法具有良好的精密度、重现性和稳定性 ,平均回收率为 10 0 .71% ,RSD=1.4 1% ;线性关系良好 ,相关系数为 0 .9999。用同样方法测定了 10个不同产地密蒙花药材中毛蕊花苷的含量 ,在 0 .79%～ 2 .30 %。结论 该分析方法快速 ,简单 ,无需对样品进行太多柱前处理 ,即可达到基线分离 ,测量结果准确 ,适用于密蒙花药材中毛蕊花苷的含量测定 。

Object To establish an HPLC method for the quantitative determination of verbascoside in Buddleja officinalis Maxim. for its quality control. Methods To determine the content of verbascoside in B. officinalis by RP HPLC. Chromatographic condition: C 18 column (250 mm×4 6 mm, 5 μm), a mobile phase of methanol acetonitrile water containing 1% acetic acid (8∶16∶76) and wavelength of 254 nm. The condition for the extraction of verbascoside was optimized by the orthogonal test as: continuous extraction by 20 times of hot 70% ethanol solution for 1 h. Results This chromatographic condition was applicable for the quantitative determination of verbascoside in B. officinalis. Verbascoside was separated successfully from other constituents, and the retention time was 14 25 min. The average recovery rate of verbascoside was 100 71% with RSD 1 41% (n=6). The correlation coefficient of standard curve was 0 999 9 (n=7). The verbascoside content in B. officinalis from ten different locations was determined to be in the range of 0 79%—2 30%. Conclusion This analytical method is proved to be sensitive, quick, simple, and accurate for the quantity determination of verbascoside with good repeatability, reproducibility, and stability. The recovery rate and the linear correlation are perfect. This RP HPLC method is reliable and recommendable for the quality control of both material and preparation of B. officinalis.