羊栖菜多糖体外抗肿瘤作用及其作用机制的研究

Studies on antitumor activities of Sargassum fusiforme polysaccharide in vitro and its mechanism

目的 通过体外抗肿瘤实验观察羊栖菜多糖 ( SFPS)对 6种不同组织肿瘤的抑制作用及其对肿瘤治疗的组织特异性 ,并揭示其作用机制。方法 采用 MTT法和集落形成实验法观察 SFPS体外抗肿瘤作用 ;采用流式细胞仪观察 SFPS对肿瘤细胞周期及细胞凋亡的影响 ;采用 Fluo- 3/ AM探针标记 ,激光共聚焦技术观测细胞内[Ca2 +]i。结果 SFPS对人胃癌细胞 SGC- 790 1和人直肠癌 COL O- 2 0 5有较好的疗效。可阻滞 SGC- 790 1人胃癌细胞由 G0 / G1 期进入 S期 ,升高细胞凋亡指数 ( APO)。可使 SGC- 790 1细胞内 [Ca2 +]i先升高后下降 ,给 Ca Cl2 后[Ca2 +]i又升高。结论 SFPS对人胃癌细胞和直肠癌细胞效果较好 ,这一作用是通过诱导肿瘤细胞凋亡达到的。SFPS诱导肿瘤细胞凋亡是通过升高肿瘤细胞内 [Ca2 +]i达到的 ,[Ca2 +]i升高时 Ca2 +来源于细胞内钙库释放。

Object To study the inhibitory effect of Sargassum fusiforme (Harv.) Setch. polysaccharide (SFPS) on six different kinds of tissue tumor by antitumor experiments in vitro, and to observe tissue speciality of tumor treatment and reveal antitumor effect mechanism. Methods MTT and colony forming methods were adopted to study the antitumor activity of SFPS in vitro. Influences of SFPS on cell cycle and apoptosis were observed. LCM was adopted to measure [Ca 2+ ]i of the cells marked by Fluo 3/AM probe. Results SFPS had content antitumor activities to SGC 7901 and COLO 205. It prevented the transforming of SCG 7901 from G 0/G 1 period to S period. And it increased the apoptosis index (APO%). Applying SFPS, [Ca 2+ ]i increased first and then decreased. Further, after giving CaCl 2, [Ca 2+ ]i increased again. Conclusion By inducing the apoptosis of tumor cells, SFPS shows content antitumor activities to SGC 7901 and COLO 205. SFPS could increase the [Ca 2+ ]i in SGC 7901. And the Ca 2+ are from cellular storage.