马尾松针叶中的挥发物质对马尾松毛虫产卵行为的影响

OVIPOSITION BEHAVIOUR OF THE PINE CATERPILLAR MOTH,DENDROLIMUS PUNCTATUS INFLUENCED BY NEEDLE VOLITALES OF PINUS MASSONIANA

前期的研究已表明马尾松毛虫雌蛾主要利用嗅觉来发现马尾松针叶并在其上产卵。借助于选择产卵试验 ,进一步研究了此种昆虫的产卵行为。与正常针叶比较 ,雌蛾对在戊烷提取过的针叶上产卵的喜好性明显地下降。联系到戊烷提取物中含有引起雌蛾触角电位反应的挥发物质的事实 ,上述结果说明戊烷提取物含有某些在雌蛾进行产卵场所定位中必不可少的挥发物。以戊烷浸过的针叶作为对照 ,雌蛾对在经α -蒎烯、香叶烯、苎烯、樟脑、里哪醇 -戊烷溶液分别浸过的针叶上产卵的喜好性没有显著的变化。然而雌蛾在经β-蒎烯 -戊烷浸泡过的针叶上的产卵量要明显地低于仅用戊烷浸过的针叶。这表明高含量 β-蒎烯的针叶能使雌蛾对其产卵喜好性显著地下降。

It has been showed that females of the pine caterpillar moth, Dendrolimus punctatus mainly use olfactory cues to locate the needle of Pinus massoniana as their oviposition site in an previous study. Oviposition behaviour in this species were further studied by oviposition choice experiments. The removal of pentane extracts from needles of P. massoniana resulted in a significant reduction of oviposition preference to extracted needles than normal ones. Considering our previous study, that pentane extracts of the needles contained volatiles eliciting female electroantennogram respones, present data implied that the volatiles were necessary for female to locate their oviposition site. Comparing with oviposition preferences to pentene-dipping needles, females did not significantly change the preference to the needles dipped with α-pinene, myrcene, limonene, camphor or linalool-pentane solution, respectively. However, females laid significantly less eggs on the needles dipped in 10% β-pinene -pentane solution than those dipped in pentane only. This fact suggested that the needles containing higher amounts of β-pinene could reduce female oviposition perference.