合成冰片对沙门氏菌的诱变作用观察

Study for mutagenic of Synthetic borneol

[目的]观察合成冰片是否有诱发沙门氏菌株发生基因突变的作用。[方法]采用鼠伤寒沙门氏菌营养缺陷型回复突变试验(Ames试验)。[结果]合成冰片在0.4～250μg/皿范围内 ,无论有或无肝脏微粒体酶(S9)活化系统 ,对菌落回变无明显影响 ,且回变菌落背景正常。[结论]合成冰片对鼠伤寒沙门氏菌突变株无致突变作用 。

To observe the mutagenicity effect of Synthetic Borneol. Ames test was used. The number of backward mutant colony of four bacterial strains induced by Synthetic Borneol at the concentration within 0.4～250 μg/plate was quantified. The results did not display a significant increase compared with that induced by dimethylsulfoxide, whether with metabolic activation system (S9_mix) or not. Moreover, the backward mutant colony had normal background. [Conclusion] Synthetic Borneol did not possess evident mutagenicity through our study.