人参二醇组皂甙减轻肾缺血再灌流性兔血清致伤肾小管细胞的作用及机制

The Effects and Mechanisms of Panaxadiol Saponins Relieveing the Injury of Renal Tubular Cells Resulting From the Rabbit Serum of Acute Renal Ischemic Reperfusion

目的 :用培养的人肾小管细胞 ,观察人参二醇组皂甙 (PDS)减轻急性缺血再灌流肾损伤性兔血清(SIR)对小管细胞的损伤作用 ,并探讨其机制。方法 :用夹闭法致兔肾缺血并提取缺血血清和对照血清 ,测定血清中的MDA、NO含量和SOD活力。不同的培养基 (普通培养基、对照血清、缺血血清以及缺血血清加PDS)分别与肾小管细胞共培养 96h ,生化法测定培养液或细胞中MDA和NO含量以及SOD、LDH、Na+ -K+ -ATP酶和Ca2 + -ATP酶活力。结果 :(1)SIR中MDA和NO含量较SC的高 ,而SOD活力则降低 ;(2 )SIR可使培养液中MDA含量、细胞内的NO含量、LDH活力显著升高 ,使细胞内的SOD、Na+ -K+ -ATP酶和Ca2 + -ATP酶活力明显降低 ,而PDS则可抑制SIR的这些效应的发挥。结论 :PDS具有减轻急性肾缺血再灌流性兔血清致肾小管细胞损伤的作用。其机制可能同增强SOD活力、减轻氧自由基和NO等的细胞毒性作用、增强ATP酶活性以维持生物膜结构和功能的完整性等作用有关。

Objective:To observe the effects and approach the mechanisms of Panaxadiol Saponins (PDS) relieveing the injury induced by the rabbit serum of acute renal ischemic reperfusion(IR).Methods:To occlude the bilateral renal arteries of rabbits then reperfusion, and acquired the serum(SIR). The rabbit serum of control (SC)were acquired in the same time. The content of alondialdehyde (MDA),Nitric oxide (NO) and the activity of Superoxide Dismute (SOD) in serum were assayed. Different culture mediums (common culture mediums、SIR、SC and SIR+PDS) were used in diverse groups. To culture by 96 hours, the content of MDA, NO and the activity of SOD, LDH, ATP ase in the culture medium or cells were detected by biochemistry method.Results:(1)There were considerable higher in content of MDA and NO in SIR than those in SC, but the activity of SOD was contrary.(2)In SIR group, the content of MDA in culture medium, the content of NO and the activity of LDH in cells were significant increased, and the activity of SOD, Na +-K +-ATP ase and Ca 2+ -ATP ase in cells were remarkable decreased, and PDS could hold back those changes.Conclusion:The injury induced by the rabbit serum of acute renal ischemic reperfusion could be relieved by Panaxadiol Saponins. A possible mechanism could be concluded as follow: allaying the toxic effect of OFR and NO, increasing the activity of SOD and ATP ase , decreasing the injury of oxidation, maintaining the integrality of plasmalemma.