中国版纳微型猪近交系内源性逆转录病毒酶活性的定量检测

The Quantity Analysis of Reverse Transcriptase in Porcine Endogenous Retrovirus Expressed in Banna Minipig Inbreed

采用 Roche公司的 RT(Reverse transcriptase,RT)检测试剂盒 ,按其操作步骤进行 ,用 HIV- 1作为试剂盒的阳性参照绘制标准曲线 ,PK15作为猪内源性逆转录病毒 (Porcine endogenous retrovirus,PERV)的阳性对照 ,定量检测 34头中国版纳微型猪近交系 (Banna minipig inbreed,BMI)血浆中表达的 PERV的逆转录酶。各猪RT结果均于 4 0 5 nm处测定 OD值 ,用 HIV- 1的实测标准曲线进行定量 (pg/ m l)。检测结果表明全部 34头猪均为阳性反应 ,但各猪血浆中有活性的酶远低于 HIV- 1,也弱于

Quantitative RT(reverse transcriptase) assay was established to detect the reverse transcriptase in plasma of thirty four Chinese Banna minipig inbreed in this work. The protocol was given in the RT kit (Roche), using HIV 1 as the positive control of the kit and supernatant of PK 15 as the PERV positive control respectively. The results show that positive reverse transcriptase reaction can be detected in the plasma of the pigs, but the levels are much lower than that of HIV 1 and lower than that of PERV in supernatant of PK 15.