无创性动态测定血糖的动物实验

Noninvasive Continuous Measurement of Blood Glucose Concentration via Animal Skin

探索一种无创性动态测定血糖的原理和建立实验方法。使用共价键固定法 ,制作葡萄糖氧化酶传感器 ,选择传感器最佳制作方法和稳定的工作条件。设立超声前、后组 ,注射葡萄糖前、后及高频、低频超声对照组。在 10只日本大耳兔的皮肤表面 ,用高频或低频超声波处理皮肤 5 m in后 ,用葡萄糖氧化酶传感器测定其微电流信号的变化。采用葡萄糖氧化酶传感器和微电流检测仪 ,可观察到经超声波处理后 ,兔皮肤表面 ,微电流信号明显增高变化(P<0 .0 1)和在注射葡萄糖后的进一步增高变化 (P<0 .0 1)。兔皮肤表面经超声波处理后 ,对葡萄糖的通透性明显增高 ,经葡萄糖氧化酶传感器转化为微电流信号的变化 ,与兔体内的葡萄糖水平呈正相关。在活体动物皮肤表面 。

In this study we deliberated over the principles and methods and then took the noninvasive continuous measurement of blood glucose concentration through the skin of rabbits.The glucose oxidase sensor was made by covalent immobilization. The best making method of sensor and stable working condition were sifted.Ten female and 10 male adult white rabbits were allocated into the groups of the ante ultrasound and post ultrasound, the injection of glucose, and the high and low frenquency ultrasounds.After the skin surface was treated by high or low frenquency ultrasound for 5 minutes on the rabbits, obvious changes( P <0.01) of post ultrasound and post injection of glucose were observed by means of glucose oxidase sensor and microcurrent apparatus.After application of ultrasound to the skin of rabbits, the penetration of glucose through the rabbit skin increased obviously. The change of microcurrent signal that was exchanged by the glucose sensor correlated positively with the concentration of glucose of rabbit body. The blood glucose can be tested by the glucose sensor on the skin surface of living animal.