双歧杆菌抗消化道逆环境特性的研究

The Study on GIT Conditions Tolerance Ability of Bifidobacterium

应用模拟胃液pH、胃液、肠液、肠胆盐环境 ,研究青春双歧杆菌、婴儿双歧杆菌、长双歧杆菌和短双歧杆菌的抗消化道逆环境特性。在pH1 5酸性条件下作用 1 5h ,存活率分别为 0 14 %、17 86%、49 0 9%和 9 5 3 %。在加有胃蛋白酶的模拟胃液中作用 1 5h ,存活率分别为 :45 3 9%、97 2 7%、84 5 3 %和 85 5 1%。在含胰蛋白酶的模拟肠液中作用 12h ,存活率分别为 :2 9 17%、47 97%、87 70 %和 77 2 7%。青春双歧杆菌、长双歧杆菌和短双歧杆菌的活菌数随胆盐浓度升高而下降 ,2 %胆盐浓度条件下作用 12h ,三者的存活率分别为 5 92 %、13 96%和 61 2 4%。而婴儿双歧杆菌在各胆盐浓度条件下 ,均有生长趋势 ,这种趋势随胆盐浓度升高而下降。研究结果表明 ,消化道逆环境对双歧杆菌的存活有不同程度的影响 ,以婴儿双歧杆菌和长双歧杆菌的耐性最好。

Characteristics on gastrointestinal (GIT) condition tolerance of B adolescentis, B infantis, B longum and B breve were investigated in simulative pH of gastric acid, gastric juice, intestinal juice and intestinal bile salt Survivability of B adolescentis, B infantis, B longum and B breve was 0 14%, 17 86%, 49 09% and 9 53% respectively after 1 5 hours in pH1 5 condition, and was 45 39%, 97 27%, 84 53% and 85 51% respectively after 1 5 hours in simulative gastric juice containing pepsin and was 29 17%, 47 97%, 87 70% and 77 27% respectively after 12 hours in simulative intestinal juice containing pancreatin The amounts of viable B adolescentis, B longum, and B breve decreased with the concentration of bile salt increased The survivability was 5 92%, 13 96%, and 61 24% respectively after 12 hours in simulative intestinal juice containing 2% bile salt The growth speed decreased with concentration of this bile salt increased The GIT conditions tolerance ability of this four Bifidobacterium strains was different The tolerance of B infantis and B longum were best among the tested strains