摘要
为探讨卵巢切除对CCl4 诱导大鼠肝纤维化形成的影响 ,采用CCl4 诱导雌性大鼠肝纤维化动物模型 ,观察卵巢切除及雌激素替代治疗 (苯甲酸雌二醇 1mg kg)对肝脏胶原沉积和I、Ⅲ型胶原蛋白表达的影响 ,并分别检测血清学标志及肝脏组织学等变化。结果显示CCl4 模型组大鼠肝脏发生典型的肝纤维化改变 ,卵巢切除组的肝脏胶原沉积更为明显 ,肝脏表达I、Ⅲ型胶原及血清肝纤维化指标也明显高于CCl4 摸型组 (P <0 0 5 ) ,而雌激素干预及替代治疗则可抑制肝纤维化的形成。表明卵巢切除加速CCl4 诱导大鼠肝纤维化的形成 ,其发生可能与卵巢分泌的雌激素对肝纤维化的抑制作用有关。
To study the effects of ovariectomy on CCl 4 induced hepatic fibrosis in rats and to investigate the suppressive effects of estrogen on liver fibrosis. Liver fibrosis was induced in ovariectomized rats by CCl 4 administration and then treated with benzoic estradiol(1mg/kg) twice a week. The expression of type I and Ⅲ collagen, the hepatic collagen synthesis and other indicators were studied. Results demonstrated that in CCl 4 group with typical liver fibrosis. ovariectomy aggravated hepatic collagen content, increased AST, ALT, hyaluronic acid(HA) and type IV collagen(CIV) in serum and enhanced expression of type I and Ⅲ collagen significantly in the fibrotic rats. Estradiol replacement therapy had an opposite effect. It was showed that ovariectomy aggravated CCl 4 induced hepatic fibrosis in rats. The mechanism may be explained by antifibrogenic effect of estrogen in liver.
出处
《基础医学与临床》
CSCD
北大核心
2003年第5期551-555,共5页
Basic and Clinical Medicine
基金
西安交通大学博士学位论文基金 (2 0 0 10 13)
