利用酶学速率法检测血清总二氧化碳

The measurement Total Carbondioxide in Serum by Kinetic Method of Enzymatic Analysis.

目的解决目前酶学终点法检测血清总CO_2偏差太大的问题。方法以速率法代替终点法,并改进标准的配制方法。结果采用以上改进措施,测量30份血清中总CO_2,并与电极法测量结果作配对t检验t=1.12。结论血清CO_2酶学速率法的准确度大大优于酶学终点法,而与电极法相当,应尽快替代目前普遍使用的酶学终点法。

Objectve To solve a problem that there is a big error in end-point method of enzymatic analysis of measuring total carbondiox-ide in serum.Methods Using kinetic method replace end-point method and improve normal compounding methods.Result Compare measure total carbondioxide in thirty share serum using kinetic method of enzymatic analysis with electrode method.The result of student test is t = 1.12. Conclusion The accuracy of kinetic method of enzymatic analysis of carbondioxide in serum is better than end-point.It is as good as electrode method. End-point method of enzymatic analysis of measuring toatl carbondioxide in serum should be replaced by kinetic method quickly.