高效液相色谱法测定延胡索中延胡索乙素的含量

Determination of dl-Tetrahydropamatine in Yanhusuo by HPLC

目的:建立高效液相色谱法测定中药材延胡索中延胡索乙素的含量。方法:以AgiLent zobax ExtendC18(250mm×4.6mm,5μm)为色谱柱,甲醇-0.1％磷酸(三乙胺调pH值至6.0)(55:45)为流动相,流速1.0mL/min,检测波长:280nm。结果:延胡索乙素在115-1376ng范围内线性关系良好,平均回收率为99.2％,RSD=1.5％(n=6)。结论:该方法简便,重现性好,可作为中药材延胡索的质量控制方法。

OBJECTIVE: An HPLC method was developed for determination of dl-Tetrahydropamatine in Yan-husuo . METHOD:The column was ODS Agilent zobax Extend C18(4. 6 ×250mm,5μm) ,mobile phase was Meth-anol - 0. 1 % phosphoric acid ( adjust pH 6. 0 with Triethylamine) (55 : 45). Detection wavelength was set at 280nm. RESULT:Linearity range was 115 - 1376ng ,The average recovery was 99. 2% ,RSD = 1. 5% (n =6). CONCLUSION: The method is accurate and can be used for quality control of Yanhusuo.