总丹酚酸和银杏叶提取物对血管平滑肌细胞保护作用的比较

Comparison of protective effects of salvianolic acids and EGb761 on vascular smooth muscle cells

目的 对丹参水溶液提取物总丹酚酸和银杏叶提取物EGb76 1对血管平滑肌细胞损伤的保护作用进行了比较研究。方法 选用培养的 10～ 2 0代大鼠血管平滑肌细胞用于实验研究。细胞用H2 O2 ( 10 0 μmol·L-1)处理 1h造成细胞氧化损伤模型 ,采用MTT方法观察总丹酚酸和EGb76 1对H2 O2 损伤细胞生存率的影响 ,同时以乳酸脱氢酶 (LDH)、丙二醛 (MDA)、一氧化氮 (NO) 3种物质变化作为指标 ,观察了总丹酚酸和EGb76 1对H2 O2 损伤血管平滑肌细胞的保护作用。结果 总丹酚酸10 0 ,10 μg·mL-1能显著提高细胞的存活率 ,而总丹酚酸 1μg·mL-1和EGb76 110 0 ,10 ,1μg·mL-1对细胞的存活率没有明显的升高作用 ;总丹酚酸 10 0 μg·mL-1,EGb76 110 0 ,10 ,1μg·mL-1均能显著降低H2 O2 损伤细胞上清液中LDH的量 ,总丹酚酸10 ,1μg·mL-1作用不显著 ;EGb76 110 0 ,10 μg·mL-1能明显降低细胞上清中MDA的量 ,EGb76 11μg·mL-1及总丹酚酸 10 0 ,10 ,1μg·mL-1作用不明显 ;总丹酚酸 10 0 ,10 ,1μg·mL-1和EGb76 110 0 ,10 μg·mL-1明显降低H2 O2 损伤引起NO的释放。结论 总丹酚酸和EGb76 1对H2 O2 损伤的血管平滑肌有保护作用 ,但作用机制似乎有所不同 。

OBJECTIVE: To study and compare the protective effects of salvianolic acids and EGb761 on the cultured rat vascular smooth muscle cells (VSMC). METHODS: The injuries of VSMC induced by hydrogen peroxide (100 μmol&middotL-1) were indicated as cell viability, release of lactic dehydrogenase (LDH) and nitride oxide (NO) and the production of malondildehyde (MBA) in medium. RESULTS: Salvianolic acids 100 and 10 μg&middotmL-1 significantly increased viability of VSMC reduced by H2O2. Salvianolic acids 100 μg&middotmL -1 and EGb761 100, 10 and 1 μg&middotmL-1 significantly decreased the release of LDH in supernatant. EGb761 100 and 10 μg &middotmL-1 apparently decreased MDA in supernatant. Salvianolic acids 100,10 and 1 μg&middotmL-1 and EGb761 100 and 10 μg&middotmL-1 also decreased the release of NO caused by H2O2. CONCLUSION Salvianolic acids and EGb761 had protective effects on VSMC, but the mechanisms remains to be further studied.