分支杆菌降解大豆甾醇侧链的发酵研究

SIDE CHAIN DEGRADATION OF BS BY MYCOBACTERIUM SP.

对分支杆菌(MycobacteriumspJY 1)降解大豆甾醇(BS)侧链至4 烯 雄甾 3,17 二酮(4 AD)和1,4 二烯 雄甾 3,17 二酮(ADD)的培养基组成、种子培养时间、通气量、投料方式及时间等发酵条件进行了研究。在投料浓度为0.3%,转化时间为168h的条件下,使4 AD(D)的转化率由原来的37%提高到50%左右。

Mycobacterium sp. which can degrade BS to 4-AD(D) was studied. The factors affecting degradation, such as seed age, construction of media ,quantity of ventilation and method of adding BS were discussed and the optimum fermentation condition was established. Based on these fermentation conditions in about 168 hours, Mycobacterium sp. can degrade 0.3% substance BS to 4-AD and the average conversion ratio of 4-AD(D) improves from 37% to about 50%.