IL-6+CD3AK细胞对MGC-803胃癌细胞株杀伤作用的体外实验研究

The killing activity of IL-6+CD3AK cells against the MGC-803 gastric carcinoma cell in vitro

目的 :培育杀伤效应更强的肿瘤杀伤细胞。方法 :IL 6 +CD3AK细胞是用人外周血单个核细胞加rIL 6、rIL 2、CD3单抗制备的 ;靶细胞用MGC 80 3胃癌细胞株。效应细胞分 5组 ,每一组又按效靶比例分 4组 ,到培养第 2 4小时和 4 8小时检测各组癌细胞的死亡情况。用四唑盐 (MTT)比色法、台盼蓝排斥法检测死亡的癌细胞 ;并用各种显微镜观察肿瘤杀伤细胞杀伤靶细胞的形态变化。结果 :显示rIL 6 +CD3AK细胞组杀伤癌细胞效应比其他组强 (P <0 0 5 ) ;随效靶比例的增加和作用时间的延长 ,杀伤效应出现增强趋势。在光镜下观察到肿瘤杀伤细胞围绕癌细胞形成玫瑰花结样结构 ,在扫描电镜下观察到肿瘤杀伤细胞和靶细胞的微绒毛呈犬牙交错状结合 ,随着时间的延长 ,靶细胞表面的微绒毛逐渐变短消失 ,核膜表面出现众多孔洞 ,以至靶细胞碎裂死亡。结论 :IL 6 +CD3AK细胞对胃癌细胞的杀伤力强于LAK(只加rIL 2 )细胞。

Objective:The killing activity of tumor killer cells against the MGC-803 gastric carcinoma cells was investigated Methods:The IL-6+CD3AK cells were prepared from the peripheral blood mononuclear cells (PBMCs) from the culture media containing rIL-6 with rIL-2 and anti-CD3Ab The other control groups were cultivated in rIL-6 only, in rIL-6 with anti-CD3ab, in rIL-2 only Each group was divided into four groups by the ratio of effect and target cells After 24 h and 48 h, the death rate of cancer cells was determined by MTT assay and trypan-blue exclusion, the morphologic changes were observed by various microscope Results:The killing activity of rIL-6+CD3AK cells was higher than those of the other groups(P<0 05) comparing with control group The group with higher ratio of effect-target cells showed the stronger killing activity The tumor killer cells revolved round cancer cells and changed into rosette The microvilli of effect-target cells had appearance of combining and formatting caniniform When the target cells were observed with time interval,cells eventually showed piecemeal death Conclusion:The killing activity of rIL-6+CD3AK was stronger than that of LAK(in rIL-2 only) cells