利用玉米芯木聚糖酶法制备低聚木糖的研究

Preparation of Xylooligosacchrides by the Xylanase B from Thermotoga maritima

该文采用源于Thermotoga maritima的木聚糖酶基因工程菌JM109(DE3)/pET-20b-xynB制备木聚糖酶液,试验结果表明,工程菌产木聚糖酶的最佳诱导条件:诱导剂乳糖浓度为25mmol/L;诱导时间为6h.酶法制备低聚木糖时,采用3%～5%的底物浓度和11.25U/g的酶用量较为适宜.TLC检测海栖热袍菌木聚糖酶水解玉米芯木聚糖的酶解产物主要为木二糖和木三糖.

The processing for preparing xylanase B using genetic modified E. coli JM109 (DE3)/ pET-20b-xynB from Thermotoga maritime is reported in this paper. For expression of xylanase using lactose as an inducer, the optimal conditions were: lactose concentration 25 mmol/L; inducing time 6 h. The rational substrate concentration was 3% ~ 5% and the proper amount of xylanase was 11.25 U/g substrate for enzymatic hydrolysis of corncob xylan. The result of TLC showed that the main components in the products were xylobiose and xylotriose.