激光预处理种子提高大豆幼苗抗冷害的机理探讨

Mechanism research of soybean seedling cold acclimation through pretreatment of soybean seeds with He-Ne laser

从能量转移、膜系统和自由基 3个方面探讨了激光预处理大豆种子提高其幼苗抗寒性的机理。研究结果表明 ,激光 (He Ne)预处理大豆种子不但明显地提高大豆种子三磷酸腺苷 (ATP)的含量 ,而且提高其幼苗 (子叶期到第一真叶期 )的抗低温胁迫的能力。处理组幼苗在 3℃～ 5℃低温胁迫下比对照组有较高的过氧化氢酶 (CAT)、过氧化物酶 (POD)、超氧化物歧化酶 (SOD)和淀粉酶 (AML)的活性及可溶性蛋白质含量。同时预处理还降低了幼苗子叶中超氧阴离子 (O2 -.)含量、丙二醛 (MDA)

How laser pretreatment can improve soybean seedlings’ cold acclimation is studied in the view of energy transformation,biomembrane integrality and oxygen free radical injury.The results show that pretreatment of soybean seeds with He-Ne laser enhance not only the seeds’ content of ATP but also seedlings’ cold acclimation during cotyledons to primary leaves stage. Pretreatment groups’ seedlings at 3℃～5℃ temperature have much higher enzyme activities and content of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD),amylase(AML) and soluble protein content than those in control. Pretreatment of soybean seeds with laser also decreases the rate of product of O 2 -.,the accumulation of MDA and the conductivity rate at low temperature.