摘要
目的 :应用基因芯片技术获取正常生育人睾丸组织与无精子症病人睾丸组织中差异表达的基因 ,并对结果进行初步分析研究。 方法 :抽提正常生育人睾丸组织与无精子症病人睾丸组织中的mRNA来制备探针 ,经过点样、杂交及洗涤后 ,通过计算机观察两者表达谱的差异情况 ,随后通过文献检索系统对其中明显上调的基因之一RAP1A进行了初步分析。 结果 :通过基因芯片筛选 ,获得 6 2 3条差异表达的基因 ,其中明显上调的基因RAP1A与人类精子调控关系密切。 结论 :基因芯片筛选正常生育人睾丸组织与无精子症病人睾丸组织差异表达的基因具有样品用量少、速度快的特性 ,可用于高通量筛选无精子症基因以及进一步的研究。
Objective: To study the differential gene expression profiles between the normal and aspermia human testes by genechips.Methods: Probes were prepared from mRNA extracted from both normal and aspermia testes and employed on Biostar H 40s genechips to detect the differential gene expression profiles. A distinctly up regulated gene RAP1A was analyzed by bibliographic retrieval. Results: Six hundred and twenty three differential expressed genes were found, among which the distinctly up regulated gene RAP1A was closely related to human sperm regulation. Conclusions: Screening the differential gene expression profiles between the normal and aspermia human testes by genechips can be used in the study of aspermia related genes.
出处
《中华男科学杂志》
CAS
CSCD
2003年第9期654-657,共4页
National Journal of Andrology
基金
陕西省人口与计划生育专项课题 (2 0 0 10 2 )
