摘要
目的 :探讨一氧化氮合酶 (NOS)抑制剂N 硝基 L 精氨酸甲酯 (L NAME)对大鼠隐睾生殖细胞凋亡的保护作用。 方法 :用 2 2dSD雄性大鼠复制单侧隐睾模型。实验分假手术组、隐睾组、隐睾 +L NAME组 [术后腹腔注射L NAME ,10mg/(kg·d) ],每组大鼠各 10只。术后 7d ,用生物素 dUTP/酶标亲和素测定法检测睾丸生殖细胞凋亡 ,用硝酸还原酶法测定睾丸组织中N0含量 ,用化学比色法测定睾丸组织中NOS活性。 结果 :术后第 7d ,与假手术组睾丸相比 ,隐睾组睾丸发生凋亡的生殖细胞数显著增加 ,隐睾 +L NAME组睾丸发生凋亡的生殖细胞数比隐睾组显著减少 (P <0 .0 1) ,隐睾 +L NAME组睾丸组织中NO含量及NOS活性与隐睾组相比显著降低 (P<0 .0 1)。 结论 :隐睾组织中NO和NOS升高是隐睾生殖细胞凋亡增加的病理机制之一 ,L NAME通过抑制NOS活性、减少NO的产生来降低睾丸组织生殖细胞的凋亡发挥其保护作用。
Objective: To explore the protective effect of nitric oxide synthase inhibitor (L NAME) on the germ cell apoptosis in the rat cryptorchid. Methods: Immature rats (22 day old Sprague Dawley) were subjected to unilateral cryptorchid. Thirty rats were divided into three groups: sham operation group (testes still in the scrotum after operation ); operation group; operation + L NAME group(given L NAME 10 mg/kg after operation, dip). Seven days after operation germ cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).Biochemical parameters (NO, NOS) were evaluated with spectrophotometric determination. Results: At the 7th day after the operation, compared with the control, the number of apoptotic germ cells in the cryptorchid testis was increased significantly, but the testis weight was decreased predominantly( P < 0.01 ). The levels of NO and NOS in the cryptorchid were significantly higher than the control. Conclusions: The levels of NO and NOS might be involved in the germ cell apoptosis in the cryptorchid; L NAME could protect the germ cell from apoptosis in experimentally cryptorchid rats by reducing the activity of NOS and reducing the level of NO in the testis.
出处
《中华男科学杂志》
CAS
CSCD
2003年第9期684-686,689,共4页
National Journal of Andrology