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志贺氏菌属各亚群菌株基因组“共有骨架”组成的分析 被引量：6

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摘要 不同原核生物在基因组组成上的差异是其生物学性状差异的基础,然而,进化中亲缘关系较近的菌群,其基因组除了含有群或株特异的基因外,还含有反映它们起源和进化痕迹的“共有骨架”结构,而这些“共有骨架”恰恰是它们基本生存能力和共有生物学性状的基础.志贺氏菌在起源和进化上与大肠杆菌极为密切,目前倾向于将二者划为同一个种.利用大肠杆菌K～12全基因组及Sf301特异性ORFs的芯片研究了志贺氏菌4个群间的基因组组成.结果显示,分别有16％～22％K-12的ORFs序列没有在志贺氏菌的基因组中被检测出,而志贺氏菌的基因组中包含至少2800个保守的ORFs,组成了其共有的“共有骨架”.进一步分析提示,这些“共有骨架”是维持肠道细菌正常生命生理活动所必需的基本组成此外,只有20％的Sf301特异性ORFs同时存在于其他3个菌株中,揭示了各菌株间基因组的异质性和菌属内的遗传多样性.
作者 刘红 彭俊平 杨剑 孙立连 陈淑霞 金奇
机构地区 病毒基因工程国家重点实验室
出处 《科学通报》 EI CAS CSCD 北大核心 2003年第23期2451-2456,共6页 Chinese Science Bulletin
基金 国家重点基础研究发展规划(批准号:G1999054105) 国家高技术研究发展计划(批准号:2001AA223011)资助项目 国际科技合作重点项目(批准号:2001AA223116) 北京市科技项目(合同号:H0102-10360119).
关键词 志贺氏菌属 大肠杆菌K-12 全基因组微阵列 骨架序列
分类号 Q933 [生物学—微生物学]
  • 相关文献

参考文献14

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共引文献17

同被引文献80

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引证文献6

二级引证文献5

科学通报
2003年 第23期

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