猪肺脏中鹦鹉热衣原体的分离

ISOLATION OF CHLAMYDIA PSITTACI IN THE LUNGS OF PIGS

从猪肺脏中分离出鹦鹉热衣原体(Chlamydia psittaci)。经SPF鸡胚卵黄囊培养分离,并经属特异性抗原和特异性单克隆抗体免疫荧光技术鉴定,从54例猪肺脏中分离出5株鹦鹉衣原体。其中40例病变肺中分离出3株,14例无明显病变肺中分离出2株。5株衣原体均从小猪肺中分离获得。

Chlamydiae psittaci were isolated from the lungs of pigs. By inoculation of the yolk sac of embryonting chicken eggs lung samples from 54 pigs were tested for chlamydiae, Chlamydiae were isolated from 5 samples: 3 islates from 40 samples of pathological lungs and 2 isolates from 14 healthy lungs and the 5 isolates all from piglets. Taxonomic classification of the isolates as C. psittaci was carried out by demonstrating the ginus specific chlamydial antigen by fluorescent antibody technic and with the aid of a fluorescein-conjugated monoclonal antibody.