侵袭性牙周炎和慢性牙周炎的龈下优势菌分析

Analysis subgingival predominant microflora of aggressive periodontitis and chronic periodontitis

目的 :分析侵袭性牙周炎 (aggressiveperiodontitis ,AgP)与慢性牙周炎 (chronicperiodontitis ,CP)的龈下优势菌群 ,为探讨牙周炎分类、病因和诊断提供实验依据。方法 :将中学生流调筛选 (16例 )及牙周病专科就诊(2 4例 )的AgP和CP患者 ,采集龈下菌斑样本 ,在厌氧菌基础培养基 (CDC)和选择性培养基 (TSBV)上培养分析。结果 :局限型AgP患者的伴放线放线杆菌 (Actinobacillusactinomycetemcomitans ,Aa)及兼性厌氧菌的检出率显著高于中度CP患者 (P <0 .0 5 ,P <0 .0 1) ,而广泛型AgP和重度CP患者的厌氧菌总数较局限型AgP和中度CP患者显著增加 (P <0 .0 5 )。结论 :局限型AgP和中度CP的龈下优势菌有明显差别 ,Aa是一个重要的危险因子。

Objective:To analyse subgingival predominant microflora of aggressive periodontitis (AgP) and chronic periodontitis (CP), and to provide experimental evidence of classification, etiology and diagnosis of periodontal diseases. Method:Subgingival plaque samples of AgP and CP from epidemiological survey of middle school students (16 cases) and clinic diagnosis of periodontal department patients (24 cases) were inoculated in non-selective medium (CDC) and selective medium(TSBV) to inspect predominant microflora.Result:When localized AgP and moderate CP patients were compared, there was a significant difference in the number of Actinobacillus Actinomycetemcomitans (Aa) positive ( p <0.05) and facultative anaerobes ( P <0.01), the total number of anaerobes in generalized AP and severe CP increased compared with localized AgP and moderate CP (P<0.05). Conclusion:Subgingival microflora in the early stage of AgP is obviously different from CP. Aa is an important risk factor for initiation of AgP.