当归注射液对全脑缺血后神经再塑影响的实验研究

The Effect of Angelica Injection on Neuronal Plasticity after Global Cerebral Ischemia

目的 :研究全脑缺血后海马区的神经再塑及当归注射液的影响。方法 :6 0只健康沙土鼠 ,双侧颈总动脉夹闭 1 0min制作全脑缺血再灌注模型 ,分为治疗组和对照组 ,前者经腹腔给予当归注射液 ,后者给予生理盐水 ,另选 6只沙土鼠作为正常组。于术后 1 6h ,1 ,2 ,3,6d采用免疫组织化学方法检测海马区生长相关蛋白 (growth associ atedprotein ,GAP 4 3)、微管相关蛋白 (microtubule associatedprotein ,MAP 2 )和突触素 (synaptophysin ,SYN)表达的变化。结果 :全脑缺血 1 0min再灌注 1 6h至 6d ,动物脑组织各区尚未见明显形态学变化 ,而MAP 2已随再灌注时间的延长表达水平逐渐降低 ,至再灌注 3d达到最低 ,再灌注 6d又轻度上升 ;GAP 4 3和SYN的表达水平则随再灌注延长而不断上升 ,至再灌注 6d达到最高 ;在各时间点 ,治疗组MAP 2、GAP 4 3和SYN的表达水平均高于对照组 (P <0 .0 5 )。结论 :全脑缺血后海马区有明显的神经再塑和结构重建 。

Objective: To study the effect of angelica injection on neuronal plasticity in hippocampus after global cerebral ischemia. Methods: 60 gerbils were used to develope global cerebral ischemia model by ligating bilateral common artery for 10 minutes and then divided into treatment group, which were administrated with angelica injection intraperitonealy,and control group that accepted normal saline. And another 6 gerbils were put into normal group without operation and treatment. At 16 h,1,2,3,6 d after operation, immunohistochemistry was used to evaluate the expression of GAP 43,MAP 2 and SYN proteins in hippocampus of animals. Results: No evident morphologic changes were detected after operation. MAP 2 expression gradually decreased,reaching its lowerest level at 3 d,and sligthly increased at 6 d. While expressions of GAP 43 and SYN kept increasing. On each stage,levels of all the three proteins expression in treatment group were higher than those in control group. Conclusion: There is apparent neuronal plasticity and reconstruction in hippocampus after global cerebal ischemia. And this process can be accelerated by angelica injection.