Survivin、caspase-3在非霍奇金淋巴瘤组织中的表达及意义

Expression of Survivin and Caspase-3 in Non-Hodgkin's Lymphoma and Their Clinical Significance

背景与目的:Survivin是新近发现的凋亡抑制因子,在肿瘤的发生中起重要作用。Survivin在多种肿瘤组织广泛表达而在正常成人组织不表达,可直接抑制caspase-3和caspase-7活性。本研究旨在通过检测survivin、caspase-3蛋白在不同恶性度非霍奇金淋巴瘤(non-Hodgkinslymphoma,NHL)组织中的表达,探讨二者与NHL发病的关系及其临床意义。方法:应用免疫组织化学EnVisionsystem法检测NHL患者组织标本survivin、caspase-3蛋白的表达。结果:Survivin及caspase-3在54例NHL中的阳性率分别为51.9%(28/54)和83.3%(45/54)。Survivin在低度恶性组NHL中的表达(19.0%,4/21)低于中-高度恶性组(72.7%,24/33),两组之间有显著性差异(P<0.05);caspase-3表达也有同样规律。Survivin及caspase-3的共同表达率为46.3%(25/54)。结论:NHL中有survivin过度表达。

BACKGROUND &OBJECTIVE: Survivin, a member of the inhibitor of apop tosis protein (IAP) family, can directly inhibit caspase-3 and caspase-7 activ ity and plays an important role in oncogenesis. This study was designed to inves tigate the expression of survivin and caspases-3 in non-Hodgkins lymphoma (N HL) with different aggressiveness and their clinical significance. METHODS: The expression of survivin and caspase-3 in 54 cases of NHL were determined with im munohistochemistry of EnVision. RESULTS: The expression rates of survivin and ca spase-3 were 51.9%(28/54) and 83.3%(45/54), respectively. The expression of s urvivin in NHL patients with low grade malignancy (19%, 4/21) was lower than th at of NHL patients with intermediate-high grade malignancy; the difference was statistically significant. The expression of caspase-3 showed the same tendency . Co-expression rate of survivin and caspase-3 was 46.3%(25/54). CONCLUSION: The expression of survivin is upregulated in NHL.