白刺组织培养技术的研究

Studies on tissue culture techniques of Nitraria tangutorum

试验选用唐古特白刺幼嫩茎段和叶片作为材料,研究白刺不同外植体的离体培养技术.结果表明,白刺带芽嫩茎是诱导丛生芽的良好外植体,而叶片是诱导愈伤组织的良好外植体;白刺的最适增殖、壮芽培养基是:MS+BA0.5mg/L+NAA1.0mg/L+GA32.0mg/L;最适生根培养基是:1/2MS+IBA0.5mg/L;愈伤组织诱导培养基是:MS+2,4-D0.5～1.0mg/L.

The infancy stem segments and leaves of Nitraria tangutorum were taken as explants and cultured in vitro to induce the plant regeneration.The results showed that the immature stems are good explants of shoot multiplication and the leaves are good explants of callus inducation;the multiplication medium is MS+6-BA 0.5 mg/L+NAA 1.0 mg/L+GA3 2.0 mg/L;the medium for rooting is 1/2 MS+IBA \{0.5 mg/L\};the callus inducing medium is MS+2,4-D 0.5~1.0 mg/L.