摘要
利用RAPD技术,采用从200个随机引物中筛选的22个随机引物对48个水蜜桃品种的基因组DNA进行扩增.通过180个扩增位点谱带的聚类,分析供试水蜜桃的系统发育;并运用特殊谱带,建立了水蜜桃的分子检索表;提出了重点保存的水蜜桃种质有冈山白、Springtime、Rebin、白凤、霞晖2号、早艳、奉化玉露.
Genomic DNAs of 48 accessions of (Amygdalus persica) were amplified by RAPD (Randomly Amplified Polymorphic DNA) with 22 primers selected from 200 arbitrary 10 decamer primers.Analyzing phylogenetic relationship through cluster of bands in 180 amplified loci.Molecular checking index and conservation of key germplasm including Okayamahaku,Spring time,Rebin,Hakuho,Xiahui No.2,Zhaoyian and Fenghuayulu were put forward according to special bands.
出处
《西北植物学报》
CAS
CSCD
2004年第1期111-119,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
武汉市晨光计划项目 985003074
华中农业大学国家作物改良重点实验室资助项目
中国科学院方向项目 KSCX2SW-320
关键词
水蜜桃
RAPD
系统发育
分子检索表
重点种质保存
honey peach
RAPD
phylogenetic relationship
molecular checking index
conservation of key germplasm
