蒙自凤仙花的离体保存

In vitro conservation of Impatiens mengtzeana

对蒙自凤仙花茎尖的离体保存研究结果表明:培养温度在12℃、18℃和24℃,每天12h光照(2000lx),添加1%～5%甘露醇的MS培养基对茎尖的生长有抑制作用。最适保存条件为12℃,甘露醇含量为3%,保存1年后存活率为100%;存活的幼苗及茎尖接种于增殖培养基,仍能正常生长、增殖和生根。

In vitro conservation of stem apex of Impatiens mengtzeana was studied in this paper.Growth was reduced by incubation at temperatures (12℃,18℃ and 24℃) and 12 h photoperiod (2 000 lx) and by adding mannitol [1%~5% w/v] to the MS medium.Addtion of mannitol (3.0% w/v) to the medium and incubation at low temperature (12℃) resulted in higher survival rate (100%) after one year of continuous storage.When surviving seedling and its stem apex were cultured on proliferation medium,they could normally multiply,root and regrowth.