己酸菌固定化与窖泥培养

Immobilization of Caproic Acid Bacteria and Culture of Pit Mud

以琼脂为固定化材料,将己酸菌种子包埋固定,制成固定化己酸菌块。大缸培养基中接种量1.5%～2%,将固定化己酸菌块悬挂于己酸菌大缸培养基中,33～35℃培养6d即成熟。固定化己酸菌细胞繁殖快,己酸产量高,产酸稳定性好,可连续发酵,连续使用5～6个轮次。将固定化己酸菌发酵液用于人工老窖泥的培养,窖泥质量优,曲酒生产第3轮次产的酒,窖香浓郁,总酯、总酸、己酸乙酯接近2～3年老窖酒的水平。(陶然)

Agar as immobilization materials was used for the immobilization of caproic acid bacteria germ to produce immobilized caproic acid bacteria mass(ICABM).The inoculation quantity in culture medium in jar was1.5%～2%,then the ICABM was hung in the culture medium,after6d culture at33～35℃,it matured.Immobilized caproic acid bacteria cells propagated rapidly and it had high output of caproic acid and acids produced had excellent stability.Besides,it could be used for continuous fermentation(continu-ous application for5～6times fermentation).The application of the fermentation liquid of immobilized caproic acid in the culture of manmade aged pit mud would produce quality pit mud.The liquor produced by the third turn had dense pit flavor and its total ester content ,total acids content and ethyl caproate content were close to the levels of the liquor of2～3years storage time.(Tran.by YUE Yang)