犬传染性喉气管炎病毒的分离及鉴定

Isolation and Preliminary Identification of Canine Adenovirus Type 2(CAV-2)

用犬肾细胞 (MDCK)从疑似犬传染性喉气管炎病毒感染犬的急性期血清中分离到 1株病毒 ,经血凝试验、免疫电镜观察、中和试验、免疫荧光抗体试验、细胞培养特征观察和回归动物试验等鉴定 ,证明所分离的病毒为犬传染性喉气管炎病毒 ,命名为 BJ- JB- 3。

The virus was isolated using the MDCK cells from serum of canine suspected to be infected by canine laryngotracheitis virus.By neutralization test,hemagglutination inhibition test and hemocyte agglomeration test,it was identified as (CAV-2).Observed by thin section immuno-electron microscopy,the virus possessed the typical adenovirus-like particles,with a spherical shape without envelope,a mean diameter of 70-80 nm.