摘要
目的 :探讨阿米洛利对大鼠肝星状细胞 (HSC)增殖的影响。方法 :将HSC T6细胞与不同浓度的阿米洛利共同培养 2 4h。MTT法观察阿米洛利对HSC T6增殖的效应 ,以乳酸脱氢酶 (LDH)法观察阿米洛利对HSC T6的毒性作用。结果 :阿米洛利浓度 >1.875 μmol·L 1 时对HSC增殖有抑制作用 (P <0 .0 1) ,1.875～ 60 μmol·L 1 未发现阿米洛利对HSC有毒性作用 (P >0 .0 5 )。结论 :阿米洛利可抑制HSC的增殖 ,其作用不是非特异性细胞毒作用所致。
Objective:To investigate the effect of amiloride on proliferation of hepatic stellate cells(HSC) of rat. Method:HSC-T6 were cultured in different concentration of amiloride for 24 hours. MTT colorimetry was used for assaying proliferation of HSC-T6. Cytotoxicity was measured by LDH colorimetry. Results:>1.875 μmol·L -1 amiloride can inhibit HSC proliferation. Conclusion:Amiloride can inhibite HSC proliferation and the effect is not due to nonspecific cytotoxicity of amiloride.
出处
《医药导报》
CAS
2004年第1期11-12,共2页
Herald of Medicine
关键词
阿米洛利
肝星状细胞
增殖
Amiloride
Hepatic stellate cell
Proliferation
