抑癌基因P16在膀胱移行细胞癌中的表达及临床意义

The expression and significance of supressor gene P16 protein in bladder transitional cell carcinoma

目的 探讨抑癌基因P16蛋白在膀胱移行细胞癌中的表达。方法 采用S -P放射免疫法检测 3 2例膀胱移行细胞癌、10例正常膀胱粘膜中P16蛋白的表达。结果 3 2例膀胱移行细胞癌中 ,P16蛋白表达总阳性率为 40 .63 % ;在膀胱移行细胞癌Ⅰ、Ⅱ、Ⅲ级中分别为 63 .64 %、3 3 .3 3 %、2 5 .0 0 %。P16蛋白表达的阳性率随病理分级增高而降低 ,Ⅰ级与Ⅱ级、Ⅲ级比较差异无显著性 (P >0 .0 5 )。结论 P16蛋白检测有助于对膀胱移行细胞癌的诊断 ;P16蛋白的缺失与膀胱移行细胞癌的发生、发展和预后有关。

Objective To study the expression of supressor gene P16 protein in bladder transitional cell carcinoma.Methods An S-P radioimmunoassay was used to detect the expression of P16 protein in 32 samples of bladder transitional cell carcinoma, and 10 samples of bladder mucosa. Results Of 32 samples of bladder transitional cell carcinoma, 40.63% of P16 protein expression was positive; and 63.64%, 33.33% and 25.00% of P16 protein were found in bladder transitional carcinoma stagesⅠ、Ⅱ、 Ⅲ, respectively. The higher the pathological staging was, the lower the positive rate of P16 protein was. In comparison of the positive rates among staging Ⅰ and staging Ⅱ、 Ⅲ, there were no statistically significant difference, P>0.05. Conclusion P16 protein can be an index as diagnosis of bladder transitional carcinoma; the deficiency of P16 protein is related to the onset, develoment and prognosis of bladder transitional carcinoma.