急性白血病患者血浆VEGF测定及其临床意义

The clinical significance of detecting plasma VEGF concentration in acute leukemia

目的 :探讨急性白血病 (AL)患者血浆血管内皮细胞生长因子 (VEGF)含量变化与临床病情的关系。方法 :用双抗体夹心酶联免疫吸附法 (ELISA)检测 5 4例AL患者血浆VEGF含量 ,同时检测骨髓涂片有核细胞中原始及幼稚白细胞数。结果 :①AL患者血浆VEGF含量 [(6 4 .76± 34.86 )ng/L]明显高于对照组 [(33.13± 11.97)ng/L](P <0 .0 1) ,且AL患者血浆VEGF升高与骨髓中原始及幼稚白细胞百分数增加呈正相关 (r =0 .4 37,P <0 .0 1) ;②初治组AL患者血浆VEGF含量 [(76 .0 1± 4 0 .18)ng/L]明显高于缓解组 [(42 .0 7± 17.75 )ng/L](P <0 .0 1) ,难治 /复发组患者血浆VEGF含量 [(78.2 1± 31.77)ng/L]又明显上升 ,与缓解组比较差异有显著性意义 (P<0 .0 1) ,而与初治组比较差异无显著性意义 (P >0 .0 5 )。结论 :①AL患者血浆VEGF含量升高与白血病细胞生长、增殖有关 ;②AL患者血浆VEGF含量与临床病情变化密切相关 ,可作为了解病情、观察疗效、判断预后的指标之一。

Objective:To investigate the relationships between plasma VEGF concentration and clinical condition in acute leukemia.Method:The plasma VEGF concentration of 54 patients with acute leukemia was detected by ELISA and the numbers of the blast cell of white blood cells were counted within nucleated cells in bone marrow.Result:①The plasma VEGF concentration of the AL group was significantly higher than that of control ( 64.76 ± 34.86 vs 33.13 ± 11.97 ng/L, P< 0.01 ) and there was linear correlation between the plasma VEGF concentration and the ratio of the blast cell of white blood cells in bone marrow (r= 0.437 , P< 0.01 ). ②The plasma VEGF concentration of the untreated group was significantly higher than that of remission group ( 76.01 ± 40.18 vs 42.07 ± 17.75 ng/L ,P< 0.01 ) and the plasma VEGF concentration of the refractory/relapsed group was increased ( 78.21 ± 31.77 ng/L), which was significantly higher than that of remission group (P< 0.01 ), but no significance between the refractory/relapsed group and the untreated group (P> 0.05 ).Conclusion:①VEGF may play a role in the growth and prliferation of leukemia cell. ②The plasma VEGF concentration is significantly associated with clinical condition, so it can be one of the indicators for learning clinical features and predicting prognosis of AL patients.