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mPEG修饰HLA抗原的方法学研究 被引量：2

Study on the Method of Modification with mPEG for HLA Antigen
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摘要 为了研究mPEG有效修饰HLA抗原,阻断HLA抗原与其相应抗体间特异性免疫反应的方法.在22℃,pH7.4的PBS介质中,采用浓度梯度法修饰淋巴细胞表面HLA抗原.结果显示经mPEG处理后的淋巴细胞表面HLA I类抗原与其相应抗体间的微量淋巴细胞毒试验为阴性.由此可以得出结论,经mPEG修饰后可以完全阻断HLA I类抗原与其相应抗体间的特异性免疫反应. To investigate the optimal method to block the specific immune reaction between HLA antigen camouflaged by mPEG and its antibody. The HLA-I antigens of lymphocytes were camouflaged by mPEG in concentration gradient at 22 ℃. The result of microlymphocytotoxicity test between HLA-I antigen of lymphocyte and its antibody were negative. It could be concluded that the specific immune reaction between HLA-I antigen of lymphocyte and its corresponding antibody could be completely blocked by the modification of mPEG-BTC.
作者 李伟 张印则 周华友 兰炯采 章扬培 张志欣
机构地区 北京红十字血液中心 第一军医大学南方医院输血科 军事医学科学院野战输血研究所
出处 《生命科学研究》 CAS CSCD 2003年第4期374-376,共3页 Life Science Research
基金 国家高技术发展规划863项目(2001AA261161)
关键词 mPEG修饰 HLA抗原 方法学 人类白细胞抗原 mPEG HLA camouflage
分类号 R392 [医药卫生—免疫学]
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参考文献7

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同被引文献12

  • 1赵刚,王芳,王春友.未成熟树突状细胞诱导协调性异种胰岛移植耐受的研究[J].中华器官移植杂志,2004,25(6):371-373. 被引量：3
  • 2赵刚,王芳,王春友.转化生长因子-β_1基因修饰树突状细胞诱导异种胰岛移植耐受[J].中华普通外科杂志,2005,20(7):428-431. 被引量：3
  • 3赵刚,王芳,王春友.抗原负载的免疫缺陷树突状细胞诱导异种胰岛移植耐受[J].中国免疫学杂志,2005,21(8):608-611. 被引量：1
  • 4Sutherland DE.Current status of beta-cell replacement therapy (pancreas and islet transplantation) for treatment of diabetes mellitus.Transplant Proc 2003; 35:1625-1627
  • 5de Vos P,van Hoogmoed CG,van Zanten J,Netter S,Strubbe JH,Busscher HJ.Long-term biocompatibility,chemistry,and function of microencapsulated pancreatic islets.Biomaterials 2003; 24:305-312
  • 6Chen AM,Scott MD.Current and future applications of immunological attenuation via pegylation of cells and tissue.BioDrugs 2001; 15:833-847
  • 7Murad KL,Gosselin EJ,Eaton JW,Scott MD.Stealth cells:prevention of major histocompatibility complex class II-mediated T-cell activation by cell surface modification.Blood 1999; 94:2135-2141
  • 8Jang JY,Lee DY,Park SJ,Byun Y.Immune reactions of lymphocytes and macrophages against PEG-grafted pancreatic islets.Biomaterials 2004; 25:3663-3669
  • 9de Vos P,van Hoogmoed CG,van Zanten J,et al.Long-term biocompatibility,chemistry,and function of microencapsulated pancreatic islets[J].Biomaterials,2003,24(2):305-312.
  • 10Dong Yun Lee,Kyungwook Yang.Optimization of monomethoxy-polyethylene glycol grafting on the pancreatic islet capsules[J].J Biomed Mater Res,2002,62(3):372-377.

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生命科学研究
2003年 第4期

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