CFU-E和其分化细胞的生物物理特性

BIOPHYSICAL CHARACTERS OF CFU-E AND ITS DIFFERENTIATION

用可诱发小鼠贫血的病毒(anemia-inducing strain friend’s virus,FVA)感染BALB/c小鼠,13 d后取其脾脏,用Ficoll-Urografin分层液(1.070)分离出红系集落形成单位(colony forming unit-erythroid.CFU-E)细胞,用Wright-Giemsa染液染色并用透射电镜进行形态学观察,在培养液加细胞因子,如促红细胞生成素(erythropoietin,EPO)、白介素3(interleukin-3,IL-3)、干细胞刺激因子(stem cell factor,SCF),诱导其分化的情况下培养12、24和36h,分别对0、12、24和36 h的细胞进行电泳率、膜的流动性和变形性的测量及红系特异转录因子GATA-1表达(0、12、24和48 h)的检测,发现CFU-E细胞随着分化培养时间增加,其电泳率不断减少,膜的流动性不断增大,细胞的变形性和取向性逐渐变好;CFU-E在0、12和24 h GATA持续高水平表达,而48 h后,其表达明显降低。

Colony Forming Unit-Erythroids (CFU-Es) were obtained from mice during the acute disease caused by anemia-inducing virus (FVA). They were induced in medium which contains EPO, BSA, IL-3 and SCF. Their biophysical characters after 0, 12, 24 and 36 h and GATA-1 expression after 0, 12, 24 and 48 h were studied. The results showed that their biophysical characters were changed regularly, and that GATA-1 expressed abundantly until CFU-E has been induced for 48 h. After 48 h, content of GATA-1 became abruptly low.