牛肝辅酶Ⅱ依赖性视黄醇脱氢酶cDNA的克隆及组织表达

cDNA Cloning and Tissue Distribution of Bovine Liver NADP(H)-dependent Retinol Dehydrogenase/Reductase

NADP(H) dependent retinol dehydrogenase/reductase (NRDR) was an important retinoic acid synthase, which was first purified from rabbit liver in 1997. In order to study the function of the NRDR gene,the full length cDNA of bovine NRDR was cloned. According to the conserved sequences of human, mouse and rabbit NRDR cDNA, a pair of primers was designed to amplify a 294 bp DNA fragment of bovine liver NRDR, and then the full length of NRDR cDNA (AF487454) was cloned by using 3′ RACE and 5′ RACE. All the cloned NRDR proteins consist of 260 amino acid residues and showed high identity among them. The tri peptide of human, mouse and rabbit NRDR C end was SRL and that of bovine NRDR C end was SHL, but both were considered to be peroxisomal target signal 1 (PTS1). RT PCR demonstrated that NRDR gene was expressed in liver, heart, lung, kidney, stomach and intestine, and was not found in pancreas, muscle, artery and skin. The full length bovine NRDR cDNA has been successfully cloned and the sequence was analyzed. It provided a reliable foundation to investigate the biological function of this protein.