加强HPLC流动相的耐用性试验研究

Rational utilization of HPLC with gradient elution technique under low-pressure

使用梯度洗脱高效液相色谱法,当以有机溶剂与缓冲盐作流动相时,应关注流动相的耐用性试验研究。

Using organic solvent and buffer solution as the mobile phase in HPLC system with gradient elution technique under low-pressure,it is essential to avoid separating out of the salt. And the durability of mobile phase should be considered.