Real time RT-PCR检测肝癌患者外周血AFP mRNA的表达水平

The Detection of AFP MRNA Expression in Peripheral Blood of Patients with Hepatocellular Carcinoma Using Real Time RT-PCR Method

目的:了解肝细胞癌患者外周血AFP mRNA的表达水平。方法;采用real time RT-PCR技术,定量检测肝细胞癌56例、慢性活动性肝炎17例、肝硬化21例和健康志愿者20例外周血AFP mRNA的表达水平。结果:肝癌56例外周血AFP mRNA的平均表达水平为34644.6±94905.4copies/μgRNA,其中29例为阳性;慢性活动性肝炎17例、肝硬化21例和健康志愿者20例外周血均未检测到AFP mRNA的表达。结论:AFP mRNA可作为血循环中存在肝癌细胞的标志物,定量检测外周血AFP mRNA是预测肝癌发生远处转移的一个可靠手段。

Objective:To detect AFP mRNA expression in peripheral blood of patients with hepatocellular carcinoma (HCC). Methods: Real time RT-PCR was used to detect AFP mRNA expression in peripheral blood from 56 patients with HCC, 17 with chronic hepatitis,21 with cirrhosis and 20 normal healthy volunteers. Results:The expression levels of AFP mRNA in peripheral blood of 56 HCC were 34644. 6±94905. 4copies/μgRNA,in which 29 were positive. AFP mRNA was not detected in 17 chronic hepatitis,21 cirrhosis and 20 normal healthy volunteers. Conclusions;AFP mRNA in peripheral blood could be used as an indicator of HCC cells in circulatory system,and be a valuable marker for predicting hematogenous spreading metastasis of HCC.