摘要
目的应用高渗灌洗液法(灌洗法)检测表达幽门螺杆菌(Hp)尿素酶B亚单位(UreB)的减毒鼠伤寒杆菌活菌重组疫苗口服免疫小鼠后的免疫应答状况。方法将能表达UreB的重组减毒鼠伤寒杆菌SL326l/pTC01-UreB口服免疫Balb/c小鼠,12周后应用灌洗法或将小鼠处死直接获取小鼠肠液,检测小鼠的肠液和血清中针对UreB的特异性IgA和IgG抗体反应。结果疫苗组小鼠的肠液和血清中可分别检测到针对UreB的特异性IgA和IgG抗体,与对照组存在显著性差异(P<0.01)。灌洗法获取的小鼠肠液中的IgA抗体含量较将小鼠处死后直接获取的肠液中的IgA抗体含量明显为高(P<0.01)。病理学检查显示疫苗组小鼠胃粘膜炎症指数无改变。结论高渗灌洗液法是获取肠道抗体的理想方法;表达HpUreB的减毒鼠伤寒杆菌SL326l/pTC01-UreB可用作抗Hp感染的口服疫苗。
Objective To determine whether attenuated Salmonella typhimurium producing Helicobacter pylori (Hp) urease subunit B (UreB) can elicit specific immune responses against Hp in mice by a lavage technique. Methods Attenuated Salmonella typhimurium producing Hp UreB immunized orally Balb/c mice twice at a 3-weeks interval. After twelve weeks, mice intestinal secretions were obtained without harm by administering a lavage solution to them intragastrically. The mice intestinal secretions of immune group were also directly washed out after the mice were killed. The antibody responses were evaluated using serum and intestinal fluid by ELISA assay. Results The multiple oral immunizations with SL326l/pTC01-UreB could induce significantly Hp-specific mucosal IgA response as well as serum IgG responses. The IgA was also consistently higher in the intestinal fluid obtained by the lavage solution than by being directly washed out. In addition, no obvious side effects for mice and no change in gastric inflammation were observed. Conclusions The attenuated Salmonella typhimurium expressing Hp UreB may be used as oral vaccine against Hp infection. And the lavage technique is an ideal method of studying mucosal immune responses.
出处
《实用医药杂志》
2003年第11期835-837,共3页
Practical Journal of Medicine & Pharmacy
关键词
幽门螺杆菌
灌洗法
鼠伤寒杆菌
疫苗
Helicobacter pylori Lavage technique Salmonella typhimurium Vaccine