脊髓火器伤后GDNFmRNA表达的实验研究

Analysis of GDNFmRNA expression after firearm wound of spinal cord in rats

目的观察脊髓火器伤后GDNFmRNA的表达变化并探讨其意义。方法利用民用射钉枪建立大鼠脊髓火器损伤模型,不同时间采用半定量RT-PCR方法,观察T12～L1段脊髓GDNFmRNA表达的变化。结果脊髓损伤前,GDNFmRNA在T12～L1段脊髓微量表达,脊髓损伤后表达逐渐减少,伤后1、4、7、10d分别减少25%、71%、82%、86%。结论脊髓GDNFmRNA表达减少,是由于失去靶组织转运GDNFmRNA所造成,这为外源性GDNF治疗脊髓火器伤提供了理论依据。

Objective To investigate the gene expression of Glial cell line-derived heurotrophic factor (GDNF) mRNA on T12～L1 spinal cord after firearm wound of spinal cord(SCI) in rats. Methods The specimen of T12～L1 spinal cord was taken respectively before and after SCI. The level of GDNF mRNA on spinal cord was observed and compared before and after SCI,using semi-quantitative RT-PCR method with β-actin as a inner consult. Results Minim expression of GDNF mRNA was found on spinal cord before SCI, but it decreased 25%,71%,82%,86% on 1st d, 4th d, 7th d and 10th d respectively. Conclusion The reduction of GDNF mRNA level on homologous segmental spinal cord would be caused by losing the support of GDNF mRNA from the target tissue through sciatic nerve, thus providing one of theory foundations for foreign GDNF to be used in treating SCI.