咪鲜安及其主要代谢物的紫外光谱吸收特性和对蝌蚪急性毒性的研究

Characters and Toxicity Towards Tadpole of Ultraviolet Spectrums of Fungicide Prochloraz and Its Major Metabolites

通过紫外光谱扫描,测定了咪鲜安(BTS40542)母体及其主要代谢物的紫外吸收光谱;在此基础上,采用静态实验法,研究了咪鲜安(BTS40542)母体及其主要代谢物对蝌蚪的急性毒性。结果表明:咪鲜安母体的紫外吸收峰的波长为204nm和270nm,咪鲜安的主要代谢物BTS44595、BTS44596、BTS45186的紫外吸收峰均只有一个,其波长分别为208nm、206nm和216nm;咪鲜安(BTS40542)及咪鲜安的主要代谢物:BTS44595、BTS44596和BTS45186对蝌蚪的96hLC50分别为:2.84mg·L-1、2.68mg·L-1、5.52mg·L-1、6.22mg·L-1和0.70mg·L-1,它们对蝌蚪的急性毒性大小顺序为:BTS45186>BTS40542>BTS44595>BTS44596;随着咪鲜安母体的降解代谢,咪鲜安母体及其主要代谢物的急性毒性与紫外吸收峰的波长都发生了明显的变化,表明二者之间存在一定的相关性;在评价咪鲜安的生态环境效应时,不仅应考虑咪鲜安母体的残留与毒性,还应重视咪鲜安主要代谢物的残留与毒性。

The ultraviolet absorbing spectrums of fungicide prochloraz (BTS 40542) and its major metabolites (BTS 44595, BTS 44596 and BTS 45186) were experimentally determined by ultraviolet spectrums' scanning in the test. Two ultraviolet absorbing peaks for BTS 40542 were tracked at the wavelengths of 204 nm and 270 nm, respectively. For BTS 44595, BTS 44596 and BTS 45186, only one ultraviolet absorbing peak was found at 208 nm, 206 nm and 216 nm, respectively. The acute toxicity of BTS 40542 and its major metabolites to tadpoles was also evaluated. 96- hour LC50 values for BTS 40542 and BTS 44595, BTS 44596 and BTS 45186 to tadpoles were measured to be 2.84 mg· L- 1, 5.52 mg· L- 1, 6.62 mg· L- 1 and 0.70 mg· L- 1, respectively, indicating that the toxicity of BTS 40542 and its major metabolites was to be: BTS 45186> BTS 40542>BTS 44595>BTS 44596. Results from this test indicated that there is a strong relationship between the toxicity of those compounds to tadpoles and the ultraviolet absorbing peak with the degradation of BTS 40542. This has consequence for the environmental risk assessment associated with fungicide BTS 40542 and its major metabolites.